Morphometric study of early placentas from rats fed a cafeteria diet
https://orcid.org/0000-0002-8464-8941
María Carolina Pustovrh
https://orcid.org/0000-0002-2909-1941
Liliana Salazar Monsalve
https://orcid.org/0000-0002-3087-8493
##plugins.themes.bootstrap3.article.details##
Introduction: The correct functioning of the placenta guarantees an adequate fetal nutritional status.
Objective: To histomorphologically characterize the early placenta of biomodels fed a cafeteria diet.
Materials and methods: Experimental study. 18 Wistar rats were sorted into control group (CTRL=10) and experimental group (CAF=8). CTRL fed with standard diet and CAF with cafeteria diet. On gestation day 12.5, 18 placentas were extracted, histochemically processed, photographed, and measured using a Leica DM750 microscope and ImageProPlus 7.0 software. Evaluated variables: central thickness, total area, thickness, and area of the placental labyrinth (ZL).
Results: Fetoplacental unit weight: CAF: 0.2368 ± 0.0068 g; CTRL: 0.2622 ± 0.0096 g; central thickness: CAF: 1727± 151 µm; CTRL: 1936± 140 µm; total placental area: CAF: 8.130 x 106 ± 0. 511 x 106 µm2; CTRL: 9.493 x 106 ± 0.587 x 106 µm2; thickness ZL: CAF: 409± 56 µm; CTRL: 492 ± 26 µm; area ZL: CAF: 1.240 x 106 ± 0.253 x 106 µm2; CTRL: 1.329 x 106 ± 0.165 x 106 µm2). No significant differences were found.
Conclusions: No alterations of placental morphometry were evidenced. It is recommended to study other variables such as degree of vascularization and specific molecular transporters.
Conflict of interest: None.
Placenta, rat, pregnancy, morphometryplacenta, rata, preñez, morfometría
1. de Barros Mucci D, Kusinski LC, Wilsmore P, Loche E, Pantaleão LC, Ashmore TJ, et al. Impact of maternal obesity on placental transcriptome and morphology associated with fetal growth restriction in mice. Vol. 44, International Journal of Obesity. 2020. p. 1087–96.
2. Lin YJ, Huang LT, Tsai CC, Sheen JM, Tiao MM, Yu HR, et al. Maternal high-fat diet sex-specifically alters placental morphology and transcriptome in rats: Assessment by next-generation sequencing. Placenta. 2019;78(March):44–53.
3. Song L, Sun B, Boersma GJ, Cordner ZA, Yan J, Moran TH, et al. Prenatal high-fat diet alters placental morphology, nutrient transporter expression, and mtorc1 signaling in rat. Obesity. 2017; 25:909–19.